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Human IL-10 ELISA Kit

Product Specifications

Catalogue N°
950.060.048 - 1 x 48 Discovery (pre-coated)
950.060.096 - 1 x 96 (pre-coated)
950.060.192 - 2 x 96 (pre-coated)
Assay Range
12.5 pg/ml - 400 pg/ml
Sensitivity
4.9 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human IL-10
Incubation
2h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
IL-10 ELISA Kit
Example of Human IL-10 Standard Curve

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BACKGROUND

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells.

In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa.

Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates . Recombinant protein expressed in E. coli thus retains all known biological activities.The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host- virus interaction.

The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

IL-10 in disease

IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni, Leishmania, Toxoplasma gondii and Trypanosoma infection. Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae, Mycobacterium tuberculosis  and Mycobacterium avium infections. High expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency.

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950.060.048 1 x 48 Discovery (pre-coated)
950.060.096 1 x 96 (pre-coated)
950.060.192 2 x 96 (pre-coated)

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