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Human IL-8 ELISA Kit

Product Specifications

Catalogue N°
950.050.048 - 1 x 48 Discovery (pre-coated)
950.050.096 - 1 x 96 (pre-coated)
950.050.192 - 2 x 96 (pre-coated)
Assay Range
31.25 pg/ml - 1000 pg/ml
Sensitivity
12.3 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human IL-8
Incubation
2h15
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
CXCL8
IL-8 ELISA Kit
Example of Human IL-8 Standard Curve

BACKGROUND

Interleukin 8 (IL-8) or CXCL8, Monocyte-Derived Neutrophil Chemotactic Factor (MDNCF), Neutrophil Activating Factor (NAF) and NAD-P1 is a chemokine secreted by monocytes, macrophages and endothelial cells. IL-8 chemoattracts and activates neutrophils.

The predominant form of IL-8 is a 8.4kDa protein containing 72 amino acid residues, which includes five additional N-Terminal amino-acids. IL-8 contains the four conserved cysteine residues present in CXC chemokines and also contains the “ELR” motif common to CXC chemokines that binds to CXCR1 and CXCR2.

Data indicate that IL-8 may participate in the pathogenesis of rheumatoid arthritis via the induction of neutrophil-mediated cartilage damage , and psoriasis. A causative involvement of IL-8 is found within a broad range of clinico-pathological conditions : adult respiratory distress syndrome, asthma, bacterial infections, bladder cancer, graft rejection and influenza infection, due to the now known biological properties of IL-8. This cytokine especially in combinations with other neutrophil activating agents, may prove helpful in the treatment of patients suffering from granulocytopenia, severe infections against which antibiotics are not effective, and immunodeficiency caused by HIV.

Version 25 - 09.22

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

950.050.048 1 x 48 Discovery (pre-coated)
950.050.096 1 x 96 (pre-coated)
950.050.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human IL-8 ELISA Kit 950.050.048 - 1 x 48 Discovery (pre-coated) 950.050.096 - 1 x 96 (pre-coated) 950.050.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders