Human IL-17A / IL-4 Dual Fluorospot Set

Product Specifications

Catalogue N°

874.092.001 - 1 x 96 Discovery (plate not included)
874.092.001P - 1 x 96 Discovery (non-sterile plate)
874.092.001S - 1 x 96 Discovery (sterile plate)
874.092.005 - 5 x 96 (plates not included)
874.092.005P - 5 x 96 (non-sterile plates)
874.092.005S - 5 x 96 (sterile plates)
874.092.010 - 10 x 96 (plates not included)
874.092.010P - 10 x 96 (non-sterile plates)
874.092.010S - 10 x 96 (sterile plates)
874.092.015 - 15 x 96 (plates not included)
874.092.015P - 15 x 96 (non-sterile plates)
874.092.015S - 15 x 96 (sterile plates)
874.092.020 - 20 x 96 (plates not included)
874.092.020P - 20 x 96 (non-sterile plates)
874.092.020S - 20 x 96 (sterile plates)

Target species

Human

Specificity

Recognizes natural human IL-17A and IL-4

Incubation

3h30 after cell stimulation

Cross Reaction

No cross reactivity with other human cytokines.

Kit Content

Diaclone Dual Fluorospot Sets include Capture antibody for cytokine 1, FITC-conjugated detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, capture antibody for cytokine 2, biotinylated detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA.

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BACKGROUND

IL-17A

Classically following antigenic stimulation and regulation by specific co-stimulatory molecules Naïve CD4+ T-cells where known to differentiate into Th1 and Th2 cells. However in recent years the identification of IL-17 and IL-23 has led to the classification of a third subset of the Th cell family, Th17 cells. These cells are classified on their ability to secrete IL-17A but not IFNg and IL-4 the main effector cytokines of Th1 and Th2 cells.

IL-17A, was originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et al. in 1993 and also called CTLA-8. IL-17A is a homodimeric glycoprotein consisting of 155 amino acids and has a molecular weight of 35 kDa.

IL-17A links innate and adaptive immunity and has both beneficial and pathological effects on the immune system. IL-17A is involved in inducing and mediating proinflammatory responses, commonly associated with allergic responses and induces the production of many other cytokines (such as IL-6, G-CSF, GM-CSF, IL-1β, TGFβ, TNFα), chemokines (including IL-8, GRO-α and MCP-1) and prostaglandins (e.g. PGE2) from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages). In-vivo studies have now indicated that IL-17A is an especially potent activator of neutrophils. IL-17A has been shown to play an important role in the host immune response to various infection and disease states, including bacterial, fungal and viral infections, autoimmune disease including psoriasis, rheumatoid arthritis (increased levels in the synovial fluid) and multiple sclerosis as well as inflammatory conditions such as Crohns disease.

IL-4

IL-4 is a lymphokine that co-stimulates the proliferation of activated B- and T-cells, augments the cytotoxic activity of lymphocytes and monocytes and enhances the functional activity of myeloid cells. Produced by mast cells, T-cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ cells into helper Th2 cells characterized by their cytokine secretion-profile that includes secretion of IL-4, IL-5, IL-6, IL-10 and IL-13 which favour a humoral immune response. In addition, IL-4 can inhibit the proliferation of TNF, IL-1 and IL-6 by macrophages.

IL-4 induces B-cell class switching to IgE and IgG1 isotypes, and up regulates MHC Class II production and CD23 expression.

IL-4 is a 15kDa globular glycoprotein containing 129 amino acid residues. The non-glycosylated form of the protein is fully biologically active.

Version 4 - 07.19

For research use only

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