Human IL-33 ELISA Kit

Product Specifications

Catalogue N°

873.020.048 - 1 x 48 Discovery (pre-coated)
873.020.096 - 1 x 96 (pre-coated)
873.020.192 - 2 x 96 (pre-coated)

Assay Range

31.25 pg/ml - 1000 pg/ml

Sensitivity

12.2 pg/ml

Target species

Human

Specificity

Recognizes both natural and recombinant human IL-33

Incubation

3h45

Sample Type

Serum, Plasma and other body fluids, Cell culture supernatants, Buffered solutions

Sample Size

100 µl (50µl for serum/plasma)

Cross Reaction

No cross reactivity with IL-1b, IL-2, IL-1a, IL-10, IL-12p70, Gp130, IL-17A, IL-23, IFNg, TNFa

Kit Content

Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.

• References
• Related products

BACKGROUND

Interleukin-33 is described as a IL1 family cytokine which have important functions in host defense, immune regulation and inflammation. It is synthesized as a 30kD precursor ; then cleaved by caspase-1 to generate a 18kD fragment, termed mature IL-33.

IL-33 was originally identified as a protein abundantly expressed in endothelial cells  of high endothelial venules (HEVs) and was previously named NF-HEV (nuclear factor in HEV). High level of IL-33 were also found in fibroblastic reticular cells of lymphoid tissues and certain types of epithelium, including skin keratinocytes and epithelial cells of stomach.

IL-33 induce T helper (Th) type 2 responses by signaling through the IL-1 receptor related protein ST2. Acts as Th2 chemotactic factor,  also promots cytokine production (such as IL-6, IL-13, IL-1b, TNFa), IL-33 has also a role in activating basophil cells and regulating their migration

It is recognized as important contributors to allergic inflammation  by eosinophils cells activation and degranulation.Also contributes to hyperresponsivness reaction observed in asthma when secreted by ephitelial cells.

Version 8 - 09.22

For research use only

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