- Catalogue N°
- 873.000.048 - 1 x 48 Discovery (pre-coated)
873.000.096 - 1 x 96 (pre-coated)
873.000.192 - 2 x 96 (pre-coated)
- Assay Range
- 31.25 pg/ml - 1000 pg/ml
- 19 pg/ml
- Target species
- Recognizes both natural and recombinant human IL-15
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 50 µl
- Cross Reaction
- No cross reaction with IL-2, IL-4, IL-7, IL-9, IL-10, IL-13, IL-21 and IFN-g
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Interleukin-15 (IL-15) is a glycoprotein of 14-15 kDa which was originally found in conditioned medium of a Rhesus monkey kidney epithelial cell line, CV-1/EBNA. IL-15 has been shown to be structurally similar to IL-2. IL-15 is mainly synthesized by macrophage/monocytes and other non-lymphoid cells. This cytokine exerts its effects through its binding to a membrane receptor composed of a private a chain (IL-15Ra) associated with the b chain of IL-2R and the common g subunit of different type I cytokine receptors. As expected, IL-2 and IL-15 share several effects such as the activation of NK cells, the induction of CD8+ T cell proliferation and the costimulation of B-cell proliferation and differentiation. However, IL-15 has additional effects, including the induction of T-cell polarization, differentiation of dendritric cells, inhibition of apoptosis of lymphoid cells and activation of polymorphonuclear leucocytes (PMNs). IL-15 synthesis is mainly regulated at a post-transcriptional level, and different stimuli, including LPS and cytokines (IFNg, TNFa) efficiently induce its production and release. IL-15 has a key role in the pathogenesis of inflammatory and malignant diseases. Abnormalities of IL-15 expression have been described in patients with rheumatoid arthritis or inflammatory bowel disease and in diseases associated with the retrovirus HIV and HTLV.
Version 5 - 07.19
For research use only
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