Rat TNF-α ELISA Kit
Product Specifications
- Catalogue N°
- 865.000.048 - 1 x 48 Discovery (pre-coated)
865.000.096 - 1 x 96 (pre-coated)
865.000.192 - 2 x 96 (pre-coated) - Assay Range
- 31.25 pg/ml - 1000 pg/ml
- Sensitivity
- 15 pg/ml
- Target species
- Rat
- Specificity
- Recognizes both natural and recombinant rat TNF-a
- Incubation
- 3h45
- Sample Type
- Serum, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other rat cytokines
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
- Synonym
- TNF-a
- TNF-alpha
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BACKGROUND
Tumor Necrosis Factor (TNFa), also known as cachectin, is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNFa circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNFa also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNFa. Due to its varied actions throughout the immune system, TNFa may play a role in the pathogenesis of many disease states.
TNFa production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNFa within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNFa is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNFa is enhanced by gamma interferon. TNFa then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1. Recent evidence has suggested an intracellular role for this peptide.
Version 24 - 09.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.