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Murine CD138 ELISA set

Product Specifications

Catalogue N°
861.060.001 - 1 x 96 Discovery
861.060.005 - 5 x 96
861.060.010 - 10 x 96
861.060.015 - 15 x 96
861.060.020 - 20 x 96
Assay Range
0.5 ng/ml - 16 ng/ml
Sensitivity
0.35 ng/ml (determined by using Diaclone pre-coated kit)
Target species
Murine
Specificity
Recognizes recombinant and natural murine soluble Syndecan-1
Incubation
From sample to end 2h45
Sample Type
Cell culture supernatant, serum, plasma
Sample Size
100 µl (50µl for serum/plasma)
Cross Reaction
No cross reactivity with other murine soluble molecule
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
Synonym
SYNDECAN-1

BACKGROUND

Syndecans are a transmembrane protein family within the heparin sulphate proteoglycan group that interact with many different molecules of the immune system through their heparin sulphate chains. The mammalian syndecan family consists of 4 proteins; syndecan 1 to 4 each encoded by very distinct genes. In adult tissues syndecan 1 (CD138) is predominantly expressed by epithelial cells and plasma cells (both normal and malignant) and currently considered the most reliable surface marker for plasma cells. In addition CD138 is also expressed on pre and immature B cells however this is regulated by IL-6 and LPS stimulation. Syndecan 1 has previously been shown to participate in cell to cell interactions, organ development, vessel formation and tissue regeneration following injury. CD138 is regularly cleaved from the membrane and as a consequence high levels of soluble CD138 are found in the blood, which can be easily detected using a CD138 specific ELISA. Via its heparin sulphate chains CD138 binds to and modulates the activity of a wide range of molecules involve in inflammation including chemokines, growth factors, selectins and other adhesion molecules.CD138 can also act as a receptor for collagen, fibronectin, thromobospondin and tenascin therefore involved in cell matrix adhesion. CD138 has been shown to mediate the binding of myeloma cells to type I collagen, and inhibits tumour cell invasion into collagen gels. As CD138 has been shown to have important effects on tumour cell growth, survival, adhesion and invasion syndecan-1 may be an important regulator in cancer biology.

Version 3 - 07.19

For research use only

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Formats Available

861.060.001 1 x 96 Discovery
861.060.005 5 x 96
861.060.010 10 x 96
861.060.015 15 x 96
861.060.020 20 x 96

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