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Anti-Human CD82 Azide Free

Product Specifications

Catalogue N°
857.790.000 - 200µg / 200µl
857.790.005 - 500µg / 500µl
Target species
Human
Specificity
Recognises the CD82 antigen, a 60 kDa protein.
Clone
TS82b
Application
Flow Cytometry
Immuno-Precipitation
Western Blot under non-reducing conditions
Hybridoma
Myeloma X63/AG8. x Balb/c spleen cells
Immunisation
Jurkat and HEL cell lines
Quantity
200µg or 500µg
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline. Sterile-filtered through 0.22 µm. Carrier and preservative free
Storage
Stable at +2-8°C for 12 months. DO NOT FREEZE.
Synonym
GR15
Anti-Human CD82 Azide Free
Samples:
• Positive cell lines RAJI 25 µg (lane 1) and 50 µg (lane 2) of total protein
• Positive cell lines HEL 25 µg (lane 3) and 50 µg (lane 4) of total protein
• Negative cell lines YT 25 µg (lane 5) and 50 µg (lane 6) of total protein
• MW : molecular weight Bio-Rad ref 161-0376 (PM)
 
Lysis buffer:
• Tris pH7.4, NaCl 150 mM, CaCl2 2H2O 1 mM, MgCl2 1 mM NaN3 0.02%, Triton X100 1%
 
Gel:
5-15% SDS polyacrylamide. Primary antibody TS82b at 1 µg/ml. Secondary antibody anti IgG1-HRP. Revelation was done by chemiluminescence using Bio-Rad Clarity kit.

Version 16 - 12.19

For research use only

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Formats Available

857.790.000 200µg / 200µl
857.790.005 500µg / 500µl

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