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Anti-Biotin Azide Free

Product Specifications

Catalogue N°
857.060.000 - 200µg / 200µl
857.060.005 - 500µg / 500µl
Specificity
Recognises biotin
Clone
B-E24
Application
ELISA
Immunohistochemistry
Western Blot
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
KLH conjugated Biotin
Quantity
200µg or 500µg (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline. Sterile-filtered through 0.22 µm. Carrier and preservative free
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Anti-Biotin Azide Free
Samples:
• Unbiotinylated IL-6 at 100 ng (lane 1)
in non-reducing conditions
• Biotinylated IL-6 at 100 ng (lane 2)
in non-reducing conditions
• MW: molecular weight Bio-Rad ref 161-0376
Gel: 5-15% SDS polyacrylamide
Revelation was done by chemiluminescence using Bio-Rad Clarity kit.

References

  • Ryan, P. E. et al., J. Biol. Chem.,2010; 285 (31): 23687-23698 - Pubmed link

Version 8 - 12.20

For research use only

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For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

857.060.000 200µg / 200µl
857.060.005 500µg / 500µl

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Anti-Biotin Azide Free 857.060.000 - 200µg / 200µl 857.060.005 - 500µg / 500µl )

Please contact us to discuss bulk orders