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Human Perforin ELISpot Kit

Product Specifications

Catalogue N°
856.141.001PC - 1 x 96 Discovery (pre-coated plate)
856.141.002PC - 2 x 96 (pre-coated plates)
856.141.005PC - 5 x 96 (pre-coated plates)
Target species
Human
Specificity
Recognizes natural human Perforin
Incubation
3h after cell stimulation
Kit Content
Diaclone Pre-coated ELISpot kits include precoated PVDF plates, Detection antibody, Alkaline phosphatase conjugate, BSA, BCIP/NBT ready-to-use substrate buffer.

BACKGROUND

Perforin is a 70kDa protein containing 534 amino acid.

Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.

Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Its role in the immune response against tumors and virus infection has been demonstrated.

Version 7 - 01.21

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

856.141.001PC 1 x 96 Discovery (pre-coated plate)
856.141.002PC 2 x 96 (pre-coated plates)
856.141.005PC 5 x 96 (pre-coated plates)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human Perforin ELISpot Kit 856.141.001PC - 1 x 96 Discovery (pre-coated plate) 856.141.002PC - 2 x 96 (pre-coated plates) 856.141.005PC - 5 x 96 (pre-coated plates) )

Please contact us to discuss bulk orders