Human Perforin ELISpot Set
Product Specifications
- Catalogue N°
- 856.141.001 - 1 x 96 Discovery (plate not included)
856.141.001P - 1 x 96 Discovery (non-sterile plate)
856.141.001S - 1 x 96 Discovery (sterile plate)
856.141.005 - 5 x 96 (plates not included)
856.141.005P - 5 x 96 (non-sterile plates)
856.141.005S - 5 x 96 (sterile plates)
856.141.010 - 10 x 96 (plates not included)
856.141.010P - 10 x 96 (non-sterile plates)
856.141.010S - 10 x 96 (sterile plates)
856.141.015 - 15 x 96 (plates not included)
856.141.015P - 15 x 96 (non-sterile plates)
856.141.015S - 15 x 96 (sterile plates)
856.141.020 - 20 x 96 (plates not included)
856.141.020P - 20 x 96 (non-sterile plates)
856.141.020S - 20 x 96 (sterile plates) - Target species
- Human
- Specificity
- Recognizes natural human Perforin
- Incubation
- 3h after cell stimulation
- Kit Content
- Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB and blocking reagent.
- Additional Information
- Spots read visually or with reader
References
- Van Gulck, E. R. et al, J. Virol,2008; 82(7): 3561-3573. - Pubmed link
Related products
- 854.950.000 - Anti-Human Perforin Azide Free
- 854.951.010 - Anti-Human Perforin FITC Conjugated
- 854.952.010 - Anti-Human Perforin PE Conjugated
- 854.953.020 - Anti-Human Perforin Unconjugated
- 854.958.010 - Anti-Human Perforin PerCP Conjugated
- 879.860.001 - Anti-Human Perforin Capture Antibody
- 879.860.002 - Anti-Human Perforin Detection Antibody
- 850.860.048 - Human Perforin ELISA Kit
- 851.860.001 - Human Perforin ELISA Set
- 856.141.001PC - Human Perforin ELISpot Kit
- 869.140.010 - Human Perforin ELISpot Pair
- 853.640.000 - Anti-Human Perforin Azide Free
- 853.620.000 - Anti-Human Perforin Azide Free
BACKGROUND
Perforin is a 70kDa protein containing 534 amino acid.
Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.
Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.
Its role in the immune response against tumors and virus infection has been demonstrated.
Version 8 - 04.20
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.