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Human Granzyme B ELISpot Set

Product Specifications

Catalogue N°
856.131.001 - 1 x 96 Discovery (plate not included)
856.131.001P - 1 x 96 Discovery (non-sterile plate)
856.131.001S - 1 x 96 Discovery (sterile plate)
856.131.005 - 5 x 96 (plates not included)
856.131.005P - 5 x 96 (non-sterile plates)
856.131.005S - 5 x 96 (sterile plates)
856.131.010 - 10 x 96 (plates not included)
856.131.010P - 10 x 96 (non-sterile plates)
856.131.010S - 10 x 96 (sterile plates)
856.131.015 - 15 x 96 (plates not included)
856.131.015P - 15 x 96 (non-sterile plates)
856.131.015S - 15 x 96 (sterile plates)
856.131.020 - 20 x 96 (plates not included)
856.131.020P - 20 x 96 (non-sterile plates)
856.131.020S - 20 x 96 (sterile plates)
Target species
Human
Specificity
Recognizes natural human Granzyme B
Incubation
3h after cell stimulation
Kit Content
Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB and blocking reagent.
Additional Information
Spots read visually or with reader

BACKGROUND

Granzyme B belongs to the granzymes family, which are exogenous serine proteinases. Granzymes are produced by cytotoxic lymphocytes (CTLs) and NK cells.

Activated cytotoxic T lymphocytes and NK cells are important effectors in immunologic defenses against tumor cell proliferation, viral infection and transplant tissue. They induce apoptosis via multiple mechanism thanks to granules exocytosis. Following adhesion of the CTL on the target cell (via CTL-receptor and antigen-presenting MHC molecules), CTLs secrete cytoplasmic granules containing granules and enzymes including granzymes and perforine (pore-forming protein). Granules are then released in the intercellular space, perforine forms a transmembrane pore. Through these pores, granzymes enter the cytosol of the target cell.

Granzyme B is the most powerful pro-apoptotic member of granzyme family. Granzyme B activates the intracellular caspases induising finally the death of the cell.

Granzymes are diagnostics markers for many disorders (viral infection, lymphoma’s and carcinoma’s, auto-immune disease and rejection of transplant).

Version 9 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

856.131.001 1 x 96 Discovery (plate not included)
856.131.001P 1 x 96 Discovery (non-sterile plate)
856.131.001S 1 x 96 Discovery (sterile plate)
856.131.005 5 x 96 (plates not included)
856.131.005P 5 x 96 (non-sterile plates)
856.131.005S 5 x 96 (sterile plates)
856.131.010 10 x 96 (plates not included)
856.131.010P 10 x 96 (non-sterile plates)
856.131.010S 10 x 96 (sterile plates)
856.131.015 15 x 96 (plates not included)
856.131.015P 15 x 96 (non-sterile plates)
856.131.015S 15 x 96 (sterile plates)
856.131.020 20 x 96 (plates not included)
856.131.020P 20 x 96 (non-sterile plates)
856.131.020S 20 x 96 (sterile plates)

Ordering options

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