- Catalogue N°
- 856.131.001 - 1 x 96 Discovery (plate not included)
856.131.001P - 1 x 96 Discovery (non-sterile plate)
856.131.001S - 1 x 96 Discovery (sterile plate)
856.131.005 - 5 x 96 (plates not included)
856.131.005P - 5 x 96 (non-sterile plates)
856.131.005S - 5 x 96 (sterile plates)
856.131.010 - 10 x 96 (plates not included)
856.131.010P - 10 x 96 (non-sterile plates)
856.131.010S - 10 x 96 (sterile plates)
856.131.015 - 15 x 96 (plates not included)
856.131.015P - 15 x 96 (non-sterile plates)
856.131.015S - 15 x 96 (sterile plates)
856.131.020 - 20 x 96 (plates not included)
856.131.020P - 20 x 96 (non-sterile plates)
856.131.020S - 20 x 96 (sterile plates)
- Target species
- Recognizes natural human Granzyme B
- 3h after cell stimulation
- Kit Content
- Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB and blocking reagent.
- Additional Information
- Spots read visually or with reader
Granzyme B belongs to the granzymes family, which are exogenous serine proteinases. Granzymes are produced by cytotoxic lymphocytes (CTLs) and NK cells.
Activated cytotoxic T lymphocytes and NK cells are important effectors in immunologic defenses against tumor cell proliferation, viral infection and transplant tissue. They induce apoptosis via multiple mechanism thanks to granules exocytosis. Following adhesion of the CTL on the target cell (via CTL-receptor and antigen-presenting MHC molecules), CTLs secrete cytoplasmic granules containing granules and enzymes including granzymes and perforine (pore-forming protein). Granules are then released in the intercellular space, perforine forms a transmembrane pore. Through these pores, granzymes enter the cytosol of the target cell.
Granzyme B is the most powerful pro-apoptotic member of granzyme family. Granzyme B activates the intracellular caspases induising finally the death of the cell.
Granzymes are diagnostics markers for many disorders (viral infection, lymphoma’s and carcinoma’s, auto-immune disease and rejection of transplant).
Version 9 - 07.19
For research use only
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