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Anti-Human IL-27 PE Conjugated

Product Specifications

Catalogue N°
855.302.019 - 100 tests / 1ml
Target species
Human
Specificity
Recognises both natural and recombinant human IL-27
Clone
B-G49
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c node cells
Immunisation
Recombinant human IL-27
Quantity
100 Tests / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG2a Kappa light chain
Format
Phosphate-buffered saline with 5 % BSA and 0.1 % sodium azide
Storage
Stable at +2-8°C for six months after reconstitution. DO NOT FREEZE.
Working Dilution
Reconstitute with 1 ml deionised water. Use 10μl to label 106 cells or 100 μl of whole blood.
Anti-Human IL-27 PE Conjugated
The dendritic cells were activated with 1μg/ml LPS, 10ng/ml IFN-gamma and 100ng/ml TNF-alpha (right) or cultured unactivated as a negative control (left) for 48 hr. 3.105 dendritic cells were labelled with 10μL of anti-IL-27 PE or a PE conjugated IgG2a isotype control (B-Z2) and evaluated by flow cytometry. The green peak represents IL-27 PE labelled cells. The black peak represents IgG2a isotype control cells

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

Interleukin IL-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits,Epstein-Barr virus-induced gene 3 (EBI3), a 33-kDa glycosylated protein, and IL-27p28, a 28-kDa peptide. IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naïve but not memory CD4+ T cells. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as WSX-1 (TCCR) and gp130. IL-27 has both pro- and anti-inflammatory properties.

Version 6 - 06.19

For research use only

Documents

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Formats Available

855.302.019 100 tests / 1ml

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Anti-Human IL-27 PE Conjugated 855.302.019 - 100 tests / 1ml )

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