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Anti-Human IL-12 p40/p70 FITC Conjugated

Product Specifications

Catalogue N°
855.131.010 - 100µg / 1ml
Target species
Human
Specificity
Recognises natural and recombinant human IL-12 p40 + p70
Clone
B-P24
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
Recombinant human IL-12
Quantity
100 µg / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline with 1 % BSA and 0.1 % sodium azide
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
It is recommended to establish the optimal dilution according to the system being used. Use 10 μl of antibody to label 5.105 cells
Synonym
p40 + p70

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

IL-12 is a pleiotropic cytokine initially called cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF) mainly produced by monocytes, macrophages and dendritic cells in response to bacterial products or upon interaction with activated T Cell.

IL-12 induces IFNg production and increases proliferation and cytotoxic activity of T and NK cells. Moreover, IL-12 induces CD4+ polarization to the Th1 phenotype that mediates immunity against intracellular pathogens.

IL-12p70 is the biologically active form and is composed of two subunits: IL-12p35 (also named IL-12A) and IL-12p40 (also named IL-12B). The p35 subunit has homology to IL-6, while p40 has homology with IL-23. The p40 subunit has been found to be expressed in a higher excess over p70. It is present in serum and plasma as a monomer, a heterodimer with IL-12p35 (biologically active IL-12p70) or heterodimer with IL-23p19 (IL-23). The subunits are genetically unrelated and are regulated independently: IL-12p40 is produced constitutively and in excess of IL-12p35 and IL-23p19.

Several studies show that IL-12p40, in monomer or homodimer form, was able to bind to IL-12R beta-1 and seem to be antagonist to IL-12p70 and IL-23 bounding.

Following these properties and the role of IL-12 in autoimmunity, IL-12p40 was described as a target in the treatment of autoimmune and systemic inflammatory diseases (Crohn’s disease, Psoriasis, multiple sclerosis).

Version 8 - 06.19

For research use only

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Formats Available

855.131.010 100µg / 1ml

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