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Anti-Human IL-1β PE Conjugated

Product Specifications

Catalogue N°
855.012.019 - 100 tests / 1ml
Target species
Human
Specificity
Recognises natural and recombinant human IL-1b
Clone
B-A15
Application
Flow Cytometry
Origin
Recombinant antibody from the original clone
Immunisation
Recombinant human IL-1b
Quantity
100 Tests / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline with 1 % BSA and 0.1 % sodium azide
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
Reconstitute with 1ml of deionized water. It is recommended to establish the optimal dilution according to the system being used. Use 10 μl of antibody to label 5.105 cells
Synonym
IL-1b
IL-1beta
Anti-Human IL-1b PE Conjugated
A typical staining pattern with the B-A15 monoclonal antibody of LPS activated MNC

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

Interleukin-1 Beta (IL-1b) is a member of the interleukin-1 family. This family consists of three structures related polypeptides. The first two are IL-1a and IL-1b, each of which has a broad spectrum of both beneficial and harmful biologic actions, and the third is IL-1-receptor antagonist, which inhibits the activities of interleukin-1.

IL-1 a and b present approximately 25% homology at the amino acid level, but the difference is in their tri dimensional structure. Two distinct receptor types have been isolated, that bind both forms.IL-1b are synthesized as a larger precursor, with a molecular weight 31kda. The molecular weight of the mature form is 17.5kDa.Unlike IL1a, the IL-1b precursor show a little or no biological activity in comparison to the mature form.

IL-1 is primarily an inflammatory cytokine. It belongs to a groups of cytokines with overlapping biologic properties (TNFa and IL-6). IL-1, TNF and IL-6 share the ability to stimulate T and B lymphocytes, increase cell proliferation, and initiate or suppress gene expression for several proteins. It exerts their effects by binding to specific receptors.

IL-1 (a and b) have similar biological properties, among them, the ability to induce fever, sleep, anorexia and hypotension. IL-1 stimulates the release of pituitary hormones, increases the synthesis of collagenases, resulting in the destruction of cartilage, and stimulates the production of prostaglandins, leading to decrease in the pain threshold. In addition IL-1 has some host-defense properties. However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine.

IL-1 has also been implicated in the destruction of beta cells of the islets of Langerhans, the growth of myelogenous leukaemia cells, and the development of atherosclerotic plaques. It is described in several diseases : sepsis syndrome, rheumatoid arthritis, inflammatory Bowel disease, acute and chronic myelogenous leukaemia, insulino-dependant diabetes mellitus, artherosclerosis.

Version 10 - 10.20

For research use only

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Formats Available

855.012.019 100 tests / 1ml

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Anti-Human IL-1β PE Conjugated 855.012.019 - 100 tests / 1ml )

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