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Anti-Human CD25 Biotinylated

Product Specifications

Catalogue N°
852.004.010 - 100 tests / 1ml
Target species
Human
Specificity
Recognises the Interleukin 2 Receptor a chain (IL-2 Ra), a 55 kDa protein
Clone
B-B10
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
PHA activated T cells
Quantity
100 Tests / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Lyophilisated in phosphate-buffered saline with 1% BSA and 0.1% sodium azide
Workshop
IV-V
Storage
Stable at +2-8°C for six months after reconstitution. For longer storage freeze aliquots.
Working Dilution
Reconstitute with 1 ml deionised water. Use 10μl to label 106 cells or 100 μl of whole blood.
Synonym
IL-2R α-Chain
Anti-Human CD25 Biotinylated
A typical staining pattern with the B-B10 monoclonal antibody of PHA activated lymphocytes

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

IL-2, one of the most important factors in the human immune system, is a potent T-cell growth factor whose major function is the activation of many cells of the immune system including T-cells, B-cells, macrophages and NK cells. These potent actions are mediated by IL-2 binding and signalling through its associated cell surface receptor IL-2R. This receptor is not expressed on normal or unstimulated lymphocytes but is quickly transcribed and expressed on T-cells following activation.

This IL-2R is a heterotrimeric protein consisting of three distinct glycopeptide subunits termed IL-2Ra (CD25) specific to IL-2R, IL-2Rb and IL-2Rg. The a and b chains are involved in binding IL-2 while the signal transduction following IL-2 binding is mediated by the g-chain along with the b chain. The IL-2Ra chain or CD25 is a type 1 transmembrane glycoprotein of 251 amino acids and 55kDa. CD25 can also be found as a soluble form in serum and tissue following enzymatic cleavage from expressing cells and can be identified as a 45KDa protein once shed from the membrane. As the expression and subsequent release of CD25 takes place following cell stimulation the presence of soluble CD25 (sCD25) in circulation is an excellent marker of T-cell activation.

A number of disease states linked to over expression of CD25 have previously been described including, autoimmune diseases, transplant rejection, chronic infection, B-cell neoplasm and various types of leukaemia and other forms of cancer. Because of this definite link between CD25 over expression and disease state many therapies for these conditions have evolved to inhibit this over expression of IL-2Ra.

More recently CD25 has become the major marker for distinguishing the CD4+CD25+ subset of T regulatory cells.

Version 6 - 06.19

For research use only

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Formats Available

852.004.010 100 tests / 1ml

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