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Human CD25 ELISA Set

Product Specifications

Catalogue N°
851.650.001 - 1 x 96 Discovery
851.650.005 - 5 x 96
851.650.010 - 10 x 96
851.650.015 - 15 x 96
851.650.020 - 20 x 96
Assay Range
68.75 pg/ml - 2220 pg/ml
Sensitivity
8 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human soluble IL-2R
Incubation
From sample to end 3h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human soluble receptors
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
Synonym
IL-2R

BACKGROUND

IL-2, one of the most important factors in the human immune system, is a potent T-cell growth factor whose major function is the activation of many cells of the immune system including T-cells, B-cells, macrophages and NK cells. These potent actions are mediated by IL-2 binding and signalling through its associated cell surface receptor IL-2R. This receptor is not expressed on normal or unstimulated lymphocytes but is quickly transcribed and expressed on T-cells following activation.

This IL-2R is a heterotrimeric protein consisting of three distinct glycopeptide subunits termed IL-2Ra (CD25) specific to IL-2R, IL-2Rb and IL-2Rg. The a and b chains are involved in binding IL-2 while the signal transduction following IL-2 binding is mediated by the g-chain along with the b chain. The IL-2Ra chain or CD25 is a type 1 transmembrane glycoprotein of 251 amino acids and 55kDa. CD25 can also be found as a soluble form in serum and tissue following enzymatic cleavage from expressing cells and can be identified as a 45KDa protein once shed from the membrane. As the expression and subsequent release of CD25 takes place following cell stimulation the presence of soluble CD25 (sCD25) in circulation is an excellent marker of T-cell activation.

A number of disease states linked to over expression of CD25 have previously been described including, autoimmune diseases, transplant rejection, chronic infection, B-cell neoplasm and various types of leukaemia and other forms of cancer. Because of this definite link between CD25 over expression and disease state many therapies for these conditions have evolved to inhibit this over expression of IL-2Ra.

More recently CD25 has become the major marker for distinguishing the CD4+CD25+ subset of T regulatory cells.

Version 8 - 07.19

For research use only

Documents

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Formats Available

851.650.001 1 x 96 Discovery
851.650.005 5 x 96
851.650.010 10 x 96
851.650.015 15 x 96
851.650.020 20 x 96

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human CD25 ELISA Set 851.650.001 - 1 x 96 Discovery 851.650.005 - 5 x 96 851.650.010 - 10 x 96 851.650.015 - 15 x 96 851.650.020 - 20 x 96 )

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