Human CD62E ELISA Set

Product Specifications

Catalogue N°

851.580.001 - 1 x 96 Discovery
851.580.005 - 5 x 96
851.580.010 - 10 x 96
851.580.015 - 15 x 96
851.580.020 - 20 x 96

Assay Range

1 ng/ml - 32 ng/ml

Sensitivity

0.5 ng/ml

Target species

Human

Specificity

Recognizes both natural and recombinant human soluble ELAM-1

Incubation

From sample to end 2h15

Sample Type

Serum, Plasma, Cell culture supernatant

Sample Size

100 µl

Cross Reaction

No cross reactivity with other human adhesion molecules

Kit Content

Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

Synonym

ELAM-1

E-Selectin

• References
• Related products

BACKGROUND

E-selectin (CD62E) is a 115 kDa glycoprotein, expressed only on endothelial cells after activation with interleukin-1 (IL-1), tumor necrosis factor-α (TNFα), or bacterial lipopolysaccharides. After stimulation of the endothelial cells, the newly synthesized E-selectin is rapidly detected, with maximum surface expression after 3-6 h and return to basal levels within 24 h. This rapid down-regulation, although poorly understood, has been explained by the release of a soluble form of E-selectin and the internalization of the molecule. This regulation of E-selectin expression could be crucial for controlling leukocyte accumulation in inflammatory responses. Several E-selectin ligands have been identified on leukocytes, but have not yet been cloned. Monoclonal antibodies (mAbs) specific for E selectin have been shown to inhibit leukocyte transmigration. It has been suggested that leukocyte binding to E-selectin on activated endothelium positively regulates CD11b (Mac-1) on leukocytes and induces increased adhesion by the ICAM-1 / Mac-1 interaction.

Version 9 - 03.21

For research use only

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