Human IL-10 ELISA Set
Product Specifications
- Catalogue N°
- 851.540.001 - 1 x 96 Discovery
851.540.005 - 5 x 96
851.540.010 - 10 x 96
851.540.015 - 15 x 96
851.540.020 - 20 x 96 - Assay Range
- 12.5 pg/ml - 400 pg/ml
- Sensitivity
- 5 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human IL-10
- Incubation
- From sample to end 2h45
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
References
- Audran, R. et al., Infect Immun., 2005; 73(12): 8017-26. - Pubmed link
- Audran, R. et al.,PLoS One,2009;4(10):e7304 - Pubmed link
- Boniface, K. et al., Clin Exp Immunol.,2007;150(3): 407-15. - Pubmed link
- Carrion, M. et al., J. Leukoc. Biol.,2016;100(6):1385-1393 - Pubmed link
- Filaci, G. et al., J. Immunol.,2007; 179(7): 4323-4334 - Pubmed link
- Ghannam, S. et al., J. Immunol.,2010; 185(1): 302-312 - Pubmed link
- Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9(3): 649 - 657 - Pubmed link
- Heemann, C. et al., Clin. Cancer Res.,2012;18 (13):3637-3647 - Pubmed link
- Popov, A. et al.,J. Immunol.,2008; 181(7): 4976-4988. - Pubmed link
- Rask, C. et al.,Clin Exp Immunol.,2013 172(2): 321-32 - Pubmed link
- Szpakowski, P. et al., J Immunol Res., 2015: 359153 - Pubmed link
- Teitelbaum, D. et al., Mult Scler.,2003; 9(6): 592-9. - Pubmed link
- Urzainqui, A. et al., J. Immunol., 2007;179(11): 7457-7465. - Pubmed link
- van Hooij, A. et al., Sci Rep.,2016; 6: 34260. - Pubmed link
- Zhang, Q. et al., Infect Immun.,2006; 74(8): 4735-43. - Pubmed link
- Fernández-Prior, Á. et al., Foods. 2021 Feb; 10(2): 227. - Pubmed link
- Kohno, K. et al., J Inflamm (Lond) 2021; 18: 2. - Pubmed link
- Sugiura, T. et al., J Immunol. 2020 Dec 15; 205(12): 3277–3290. - Pubmed link
- Schindler, C. R. et al., J Clin Med. 2020 Jun; 9(6): 1667. - Pubmed link
- Kordulewska, N.K. et al., Int J Mol Sci. 2021 Dec; 22(24): 13634. - Pubmed link
- Kowalewicz-Kulbat, M. et al., Vaccines (Basel) 2021 Mar; 9(3): 277. - Pubmed link
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BACKGROUND
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells.
In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa.
Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities.The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host- virus interaction.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
IL-10 in disease
IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni , Leishmania , Toxoplasma gondii and Trypanosoma infection. Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae , Mycobacterium tuberculosis and Mycobacterium avium infections. High expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency.
Version 14 - 02.22
For research use only
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