Human Perforin ELISA Kit

Product Specifications

Catalogue N°

850.860.048 - 1 x 48 Discovery (pre-coated)
850.860.096 - 1 x 96 (pre-coated)
850.860.192 - 2 x 96 (pre-coated)

Assay Range

62.5 pg/ml - 2000 pg/ml

Sensitivity

40 pg/ml

Target species

Human

Specificity

Recognizes natural human Perforin

Incubation

2h45

Sample Type

Supernatant, Buffered Solutions, Not suitable for use with serum and plasma.

Sample Size

100 µl

Cross Reaction

No cross reactivity with Trail, TRAIL R3, TRAIL R4, Granzyme B, TNFα, IFNγ, Fas, Fas L, IL-1b, IL-2

Kit Content

Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.

• References
• Related products

BACKGROUND

Perforin is a 70kDa protein containing 534 amino acid.

Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.

Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Its role in the immune response against tumors and virus infection has been demonstrated.

Version 14 - 11.21

For research use only

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