Human CD14 ELISA kit

Product Specifications

Catalogue N°

850.780.048 - 1 x 48 Discovery (pre-coated)
850.780.096 - 1 x 96 (pre-coated)
850.780.192 - 2 x 96 (pre-coated)

Assay Range

6.87 ng/ml - 220 ng/ml

Sensitivity

6 ng/ml

Target species

Human

Specificity

Recognizes both natural and recombinant human soluble CD14

Incubation

3h45

Sample Type

Serum, Plasma, Cell culture supernatant

Sample Size

100 µl

Cross Reaction

No cross reactivity with other human soluble molecules

Kit Content

Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.

• References
• Related products

BACKGROUND

CD14 is described as the marker molecule for monocytes and macrophages and as an important component of the innate immune system. It exists in two forms : mCD14 a 55 kDa protein anchored to the membrane by a glycosylphosphoinositol tail (GPI)  and two soluble serum proteins (sCD14) with or without the GPT anchor. The mCD14 is critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. CD14 is strongly expressed on cells of the myelomonocytic lineage such as monocytes and osteoclast progenitors and at a lower level on macrophages, Langerhans cells, granulocytes and microglia.

Increased sCD14 levels are associated with inflammatory infectious diseases or allergy.

CD14 is involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. CD14 is a useful marker for enumeration of monocytes or osteoclast progenitors in peripheral blood and for monocytic and myelomonocytic acute myeloid leukaemia.

Version 17 - 02.22

For research use only

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