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Human CD95 ELISA kit

Product Specifications

Catalogue N°
850.740.048 - 1 x 48 Discovery (pre-coated)
850.740.096 - 1 x 96 (pre-coated)
850.740.192 - 2 x 96 (pre-coated)
Assay Range
93.75 pg/ml - 3000 pg/ml
Sensitivity
47 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant soluble human Fas
Incubation
1h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human soluble molecules
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
APO-1
FAS
CD95 ELISA Kit
CD95 ELISA Kit


BACKGROUND

Fas (APO-1, CD95) is a transmembrane protein belonging to the TNF receptor family, capable of mediating apoptotic death of transformed cells as well as activated human T cells. The cell death signal can be induced by anti-Fas antibodies or by the Fas (Fas-L) ligand belonging to the TNF family. The in vivo expression of these two Fas and Fas-L molecules is differently regulated since the expression of Fas is pleiotropic whereas Fas-L is rather expressed in immunity sites (testes, ovaries, brain, eye) and by activated T cells. Tumor cells regardless of their tissue origin may also express Fas-L (pathological).

Genetic and immunological studies have shown that the Fas system is on the one hand involved in the process of peripheral clonal deletion allowing the maintenance of self-tolerance, on the other hand that it regulates the amplitude and duration of immune responses. and finally that it represents one of the mechanisms of lysis of the target cells in the antiviral cytotoxic T response.

When Fas-dependent apoptosis can not occur, due to genetic mutations in the Fas or Fas-L genes, a lymphoproliferative syndrome and autoimmune manifestations are observed in humans and mice. Conversely, an exacerbation of the Fas system may be responsible for tissue destruction. For example, the single mouse injection of anti-Fas antibodies induces massive liver destruction and mouse death within a few hours, leading to the hypothesis of Fas involvement in fulminant hepatitis.

Version 13 - 02.20

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

850.740.048 1 x 48 Discovery (pre-coated)
850.740.096 1 x 96 (pre-coated)
850.740.192 2 x 96 (pre-coated)

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