Human CD141 ELISA kit
Product Specifications
- Catalogue N°
- 850.720.048 - 1 x 48 Discovery (pre-coated)
850.720.096 - 1 x 96 (pre-coated)
850.720.192 - 2 x 96 (pre-coated) - Assay Range
- 0.625 ng/ml - 20 ng/ml
- Sensitivity
- 0.31 ng/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human soluble thrombomodulin
- Incubation
- 1h45
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl (50µl for serum/plasma)
- Cross Reaction
- No cross reactivity with other human soluble molecules
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
- Synonym
- Thrombomodulin
References
- Akil, A. et al., Biol Blood Marrow Transplant.,2015; 21(10): 1739-45. - Pubmed link
- Bijkerk, R. et al.,Transplant Direct. 2017 Jun 19;3(7):e174. - Pubmed link
- Budzyn, M. et al.,Biomed Res Int. 2017;2017:2791082 - Pubmed link
- Dane, M. J. C. et al., Clin. J. Am. Soc. Nephrol., 2014;9(4): 698-704 - Pubmed link
- De Leeuw K. et al., Ann. Rheum. Dis, 2005; 64(5): 753 - 759 - Pubmed link
- De Leeuw, K. et al., Lupus,2008; 17(11): 1010-1017. - Pubmed link
- De Leeuw, K. et al., Lupus,2006; 15(10): 675-682. - Pubmed link
- Geft, D. et al.,PLoS One,2008; 3(9): e3238 - Pubmed link
- Hettema, M. E. et al.,Clin Rheumatol.,2009; 28(7): 825-33 - Pubmed link
- Jie, K. E. et al., Heart, 2011; Jan(97):60-65 - Pubmed link
- Johansson, P. I. et al.,PLoS One,2015; 10(3): e0120914 - Pubmed link
- Kuhn, E. W. et al.,Biomed Res Int., 2015: 256905 - Pubmed link
- Li, J.-Y. et al., Arterioscler Thromb Vasc Biol., 2011;31(11):2518-2525 - Pubmed link
- Luft, T. et al., Blood, 2011; 118: 1685 - 1692 - Pubmed link
- Mok, C. C. et al.,Lupus,2013 ; 22(14): 1470-1478 - Pubmed link
- Nienhuis, H. L. et al.,Arthritis Res Ther.,2010;12(5): R181. - Pubmed link
- Paunel-Görgülü, A. et al.,Sci Rep.,2017;Dec 12;7(1):17421 - Pubmed link
- Robak, M. et al.,Med Oncol.2012; 29(5): 3574-80 - Pubmed link
- Ronsholt, F. F. et al.,BMC Infect Dis.,2015; 15: 388 - Pubmed link
- Skrabal, C. A. et al., J Thorac Cardiovasc Surg.,2006; 132(2): 291-6. - Pubmed link
- Teraa, M. et al., PLoS One,2013;8(1): e55592 - Pubmed link
- Wittwer, T. et al.,J Cardiothorac Surg.,2011; 6: 142. - Pubmed link
- Yano, Y. et al., J Clin Endocrinol Metab.,2003; 88(2): 736-41. - Pubmed link
- Zhang, T. et al.,PLoS One,2014; 9(11): e111992 - Pubmed link
- Berthelsen, RE. Et al., Crit Care. 2019 Sep 5;23(1): 301 - Pubmed link
- Damén, T. et al., Acta Anaesthesiol Scand. 2021 Feb; 65(2): 220–227. - Pubmed link
- Florijn, B. W. et al., Sci Rep. 2020; 10: 13945. - Pubmed link
- Krocker, J.D. et al., Int J Mol Sci. 2022 Jun; 23(11): 6213. - Pubmed link
- Yuan, L. et al., ERJ Open Res. 2022 Apr; 8(2): 00652-2021. - Pubmed link
- Schønemann-Lund, M. et al., Crit Care. 2022; 26: 33. - Pubmed link
Related products
- 854.580.000 - Anti-Human CD141 Azide Free
- 854.581.010 - Anti-Human CD141 FITC Conjugated
- 854.583.020 - Anti-Human CD141 Unconjugated
- 879.720.001 - Anti-Human CD141 Capture Antibody
- 879.720.002 - Anti-Human CD141 Detection Antibody
- 851.690.001 - Human CD141 ELISA Set
- 853.850.000 - Anti-Human CD141 Azide Free
BACKGROUND
CD141 , also known as Thrombomodulin or BDC-A is an transmembrane protein expressed predominantly on the surface of vascular endothelial cells, but also on other cell types such as macrophages, monocytes, subpopulation of myeloid dendritic cells and keratinocytes. Thrombomodulin is a type 1 membrane glycoprotein of 74kDa consisting on a single chain with 6 Epidermal Growth Factor (EGF)-like domains critical for Thrombin and proteine C binding, and four other domains (an N-terminal C-type lectin domain, a highly glycosylated serine/threonine-rich domain, a transmembrane segment, and a short C-terminal cytoplasmic tail. It acts as an important component in the anti-coagulation and fibrinolysis system : cofactor for active thrombin, the thrombomodulin-thrombin complex activates protein C to degrade coagulation factors, inhibiting coagulation and fibrinolysis.
When vascular endothelial cells injury occurs, proteolytic cleavage release multiple fragments of thrombomodulin in circulation, so it represents a good marker for endothelial cell damage in pathologies such as cardiovascular disease, acute coronary syndrome, pulmonary thromboembolism or severe hemorrhage. Thrombomodulin has been described as an anticoagulant effect protein, and its therapeutical potential for preventing deep vein thrombosis and its use in the treatment of disseminated intravascular coagulation is under demonstrated in preliminary data.
More recently, other biological effects beyond anticoagulation have been demonstrated such as an anti-inflammatory , cell adhesion and proliferation regulation It is also demonstrated that thrombomodulin may be able to suppress the metastatic capacity of tumor cells, and hence have a protective role in many types of malignancies.
Version 19 - 09.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.