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Human CD141 ELISA kit

Product Specifications

Catalogue N°
850.720.048 - 1 x 48 Discovery (pre-coated)
850.720.096 - 1 x 96 (pre-coated)
850.720.192 - 2 x 96 (pre-coated)
Assay Range
0.625 ng/ml - 20 ng/ml
Sensitivity
0.31 ng/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human soluble thrombomodulin
Incubation
1h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl (50µl for serum/plasma)
Cross Reaction
No cross reactivity with other human soluble molecules
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
Thrombomodulin
CD141 ELISA Kit
CD141 ELISA Kit

BACKGROUND

CD141 , also known as Thrombomodulin or BDC-A is an transmembrane protein expressed predominantly on the surface of  vascular endothelial cells, but also on other cell types such as macrophages, monocytes, subpopulation of myeloid dendritic cells and keratinocytes. Thrombomodulin is a type 1 membrane glycoprotein of 74kDa consisting on a single chain with 6 Epidermal Growth Factor (EGF)-like domains critical for Thrombin and proteine C binding, and four other domains (an N-terminal C-type lectin domain, a highly glycosylated serine/threonine-rich domain, a transmembrane segment, and  a short C-terminal cytoplasmic tail. It acts as an important component in the anti-coagulation and fibrinolysis system : cofactor for active thrombin, the thrombomodulin-thrombin complex activates protein C to degrade coagulation factors, inhibiting coagulation and fibrinolysis.

When vascular endothelial cells injury occurs, proteolytic cleavage release multiple fragments of thrombomodulin in circulation, so it represents a good marker for endothelial cell damage in pathologies such as cardiovascular disease, acute coronary syndrome, pulmonary thromboembolism or severe hemorrhage. Thrombomodulin has been described as an anticoagulant effect protein, and its therapeutical potential for preventing deep vein thrombosis and its use in the treatment of disseminated intravascular coagulation is under demonstrated in preliminary data.

More recently, other biological effects beyond anticoagulation have been demonstrated such as an anti-inflammatory , cell adhesion and proliferation regulation It is also demonstrated that thrombomodulin may be able to suppress the metastatic capacity of tumor cells, and hence have a protective role in many types of malignancies.

Version 15 - 07.19

For research use only

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Formats Available

850.720.048 1 x 48 Discovery (pre-coated)
850.720.096 1 x 96 (pre-coated)
850.720.192 2 x 96 (pre-coated)

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