Human IL-1α ELISA Kit
Product Specifications
- Catalogue N°
- 850.005.048 - 1 x 48 Discovery (pre-coated)
850.005.096 - 1 x 96 (pre-coated)
850.005.192 - 2 x 96 (pre-coated) - Assay Range
- 31.25 pg/ml - 1000 pg/ml
- Sensitivity
- 10 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human IL-1a
- Incubation
- 3h45
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
- Synonym
- IL-1a
- IL-1alpha
References
- Mok, C. F. et al., Evid Based Complement Alternat Med.,2013; 2013: 792840 - Pubmed link
- Rasool, M. et al., PLoS One,2016; 11(11): e0167149. - Pubmed link
- Loo, W. T. et al.,J Transl Med.,2012; 10 Suppl 1: S8 - Pubmed link
- Colarusso, C. et al., Biomedicines. 2021 Dec; 9(12): 1931. - Pubmed link
- Zerbinati, N. et al., Life (Basel) 2021 May; 11(5): 406. - Pubmed link
- Zerbinati, N. et al., Molecules. 2021 Dec; 26(24): 7592. - Pubmed link
- Colarusso, C. et al., Front Immunol. 2022; 13: 934264. - Pubmed link
- Sommatis, S. et al., Molecules. 2022 Feb; 27(4): 1255. - Pubmed link
BACKGROUND
In fact, there is not one molecule, but one family of IL-1 molecules comprising 11 members, the two most studied forms of interleukin-1 being:
IL-1α
IL-1β, its main form, involved in the reaction cascade comprising different molecules described under the name of inflammasome.
These two forms of IL-1 generally bind to the same cellular receptor, which is composed of two related but non-identical subunits, which transmit intracellular signals via a pathway mainly shared with other receptors, such as those of the Toll1 family (innate immunity receptors) and the IL-18 receptor. Stimulation of the receptor results in the secretion or synthesis of several inflammatory mediators.
The IL-1 receptor antagonist, IL-1Ra, binds to the same membrane receptor as IL-1, and prevents IL-1 from sending its signal to the cell. It is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. It is commercially produced as anakinra, which is a recombinant human form of IL-1Ra. Interleukin 1 (IL-1) is a cytokine secreted by macrophages, monocytes and dendritic cells. It plays a preeminent role in the inflammatory response of the body to infections.
Version 10 - 09.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.