Rat IL-4 ELISA Set
Product Specifications
- Catalogue N°
- 872.020.001 - 1 x 96 Discovery
872.020.005 - 5 x 96
872.020.010 - 10 x 96
872.020.015 - 15 x 96
872.020.020 - 20 x 96 - Assay Range
- 31.25 pg/ml - 1000 pg/ml
- Sensitivity
- 15 pg/ml
- Target species
- Rat
- Specificity
- Recognizes both natural and recombinant rat IL-4
- Incubation
- From sample to end 4h45
- Sample Type
- Serum, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other rat cytokines
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
References
- Mirza, S. et al., Cell Stress Chaperones,2006; 11(4): 364-78. - Pubmed link
- Schif-Zuck S. et al., J. Immunol., 2005, 174(7): 4307 - 4315 - Pubmed link
- Schif-Zuck, S. et al., J Immunol.,2006; 177(11): 8241-7. - Pubmed link
- Slack, L. K. et al.,Cell Stress Chaperones,2007; 12(1): 71-82. - Pubmed link
Related products
- 855.030.000 - Anti-Human IL-4 Azide Free
- 855.172.010 - Anti-Human IL-4 PE Conjugated
- 879.020.001 - Anti-Human IL-4 Capture Antibody
- 879.020.002 - Anti-Human IL-4 Detection Antibody
- 856.011.001 - Human IL-4 ELISpot Set
- 660.020.096 - Murine IL-4 ELISA Kit
- 850.890.048 - Human IL-4 High Sensitivity ELISA Kit
- 865.020.048 - Rat IL-4 ELISA Kit
- 950.020.048 - Human IL-4 ELISA Kit
- 851.510.001 - Human IL-4 ELISA Set
- 856.011.001PC - Human IL-4 ELISpot Kit
- 869.010.010 - Human IL-4 ELISpot Pair
- 855.170.000 - Anti-Human IL-4 Azide Free
- 853.800.000 - Anti-Human IL-4 Azide Free
- 858.000.005 - Accessory Pack for ELISA set
BACKGROUND
IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M.
Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4.
IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFNg, and lymphotoxin (TNFb). Apart from T cells, it has been shown that mast cells can produce IL-4.
IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells.
In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.
Version 7 - 07.19
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.