Human TNF-α ELISA Set
Product Specifications
- Catalogue N°
- 851.570.001 - 1 x 96 Discovery
851.570.005 - 5 x 96
851.570.010 - 10 x 96
851.570.015 - 15 x 96
851.570.020 - 20 x 96 - Assay Range
- 25 pg/ml - 800 pg/ml
- Sensitivity
- 10 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human TNF-a
- Incubation
- From sample to end 2h45
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
- Synonym
- TNF-a
- TNF-alpha
References
- Filaci, G. et al., J. Immunol.,2007; 179(7): 4323-4334 - Pubmed link
- Fornander, L. et al.,PLoS One,2013; 8(12): e83089 - Pubmed link
- Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9(3): 649 - 657 - Pubmed link
- Harel, E. et al., PLoS One,2011; 6(9): e24202 - Pubmed link
- Noizat-Pirenne, F. et al., Haematologica,2007; 92(12): e132-135. - Pubmed link
- Popov, A. et al., J Clin Invest.,2006; 116(12): 3160-70. - Pubmed link
- Popov, A. et al.,J. Immunol.,2008; 181(7): 4976-4988. - Pubmed link
- Sabour Alaoui, S. et al.,PLoS One,2012;7(3): e33609 - Pubmed link
- Zhang, Q. et al., Infect Immun.,2006; 74(8): 4735-43. - Pubmed link
- Daull, P. et al., Mol Vis. 2018 Jul 20;24:459-470 - Pubmed link
- Fernández-Prior, Á. et al., Foods. 2021 Feb; 10(2): 227. - Pubmed link
- Kordulewska, N. K. et al., Nutrients. 2021 Jan; 13(1): 123. - Pubmed link
- Steen-Louws, C. et al., Pharm Res. 2020; 37(2): 17. - Pubmed link
- Kordulewska, N.K. et al., Int J Mol Sci. 2021 Dec; 22(24): 13634. - Pubmed link
- Mörs, K. et al., Dis Markers. 2021; 2021: 6622701. - Pubmed link
- Servaas, N.H. et al., Int J Mol Sci. 2021 May; 22(9): 4365. - Pubmed link
- Kordulewska, N. et al., J Inflamm Res. 2022; 15: 1501–1519. - Pubmed link
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BACKGROUND
Tumor necrosis factor-alpha (TNF-α, also known as TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, cachexin, cachectin or TNFSF2) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction.
It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFα is a member of the TNF superfamily, consisting of various transmembrane proteins with a homologous TNF domain
TNF alpha plays a proinflammatory role during a tissue injury or a lesion, sometimes by himself or with mediators like interleukins 1 and 6.
TNF alpha has a cellular intervention where it uses lymphocytes and neutrophils but also permits tissues to remodel and repair.
TNF alpha has a positive action and will protect the body if the immune system is functioning properly. However, if the Tnf-alpha levels are altered it can lead to serious pathologies such as Behçet decease (an inflammation of the blood vessels).
TNF alpha is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.
Version 14 - 09.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.