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Human IL-4 ELISA Kit

Product Specifications

Catalogue N°
950.020.048 - 1 x 48 Discovery (pre-coated)
950.020.096 - 1 x 96 (pre-coated)
950.020.192 - 2 x 96 (pre-coated)
Assay Range
1.1 pg/ml - 35 pg/ml
Sensitivity
0.7 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human IL-4
Incubation
3h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
IL-4 ELISA Kit
Example of Human IL-4 Standard Curve

References

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BACKGROUND

IL-4 is a lymphokine that co-stimulates the proliferation of activated B- and T-cells, augments the cytotoxic activity of lymphocytes and monocytes and enhances the functional activity of myeloid cells . Produced by mast cells, T-cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ cells into helper Th2 cells characterized by their cytokine secretion-profile that includes secretion of IL-4, IL-5, IL-6, IL-10 and IL-13 which favour a humoral immune response. In addition, IL-4 can inhibit the proliferation of TNF, IL-1 and IL-6 by macrophages.

IL-4 induces B-cell class switching to IgE and IgG1 isotypes, and up regulates MHC Class II production and CD23 expression.

IL-4 is a 15kDa globular glycoprotein containing 129 amino acid residues. The non-glycosylated form of the protein is fully biologically active.

Version 28 - 09.22

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Formats Available

950.020.048 1 x 48 Discovery (pre-coated)
950.020.096 1 x 96 (pre-coated)
950.020.192 2 x 96 (pre-coated)

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