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Murine IL-10 ELISA Kit

Product Specifications

Catalogue N°
860.030.048 - 1 x 48 Discovery (pre-coated)
860.030.096 - 1 x 96 (pre-coated)
860.030.192 - 2 x 96 (pre-coated)
Assay Range
31.25 pg/ml - 1000 pg/ml
Sensitivity
20 pg/ml
Target species
Murine
Specificity
Recognizes both natural and recombinant murine IL-10
Incubation
3h45
Sample Type
Serum, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other murine cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
mIL-10 ELISA Kit
Example of Murine IL-10 Standard Curve

BACKGROUND

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates.

Recombinant protein expressed in E. coli thus retains all known biological activities. The IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The murine IL-10 exhibits strong DNA and amino acid sequence homology to the human IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.

Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Version 12 - 11.21

For research use only

Documents

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Formats Available

860.030.048 1 x 48 Discovery (pre-coated)
860.030.096 1 x 96 (pre-coated)
860.030.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Murine IL-10 ELISA Kit 860.030.048 - 1 x 48 Discovery (pre-coated) 860.030.096 - 1 x 96 (pre-coated) 860.030.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders