Human IL-10 High Sensitivity ELISA Kit
Product Specifications
- Catalogue N°
- 850.880.048 - 1 x 48 Discovery (pre-coated)
850.880.096 - 1 x 96 (pre-coated)
850.880.192 - 2 x 96 (pre-coated) - Assay Range
- 1.56 pg/ml - 50 pg/ml
- Sensitivity
- 0.98 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human IL-10
- Incubation
- 3h00
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone High Sensitivity ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
References
- Morandi, F. et al., J Immunol Res., 2015: 718975 - Pubmed link
- Piccoli, L. et al., Clin Dev Immunol., 2012: 483935 - Pubmed link
- Stępień, E. et al., Cancers (Basel) 2021 Aug; 13(16): 3981. - Pubmed link
- Adamczak, R. et al., Int J Immunopathol Pharmacol. 2022 Jan-Dec;36:3946320221111134. - Pubmed link
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BACKGROUND
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells.
In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa.
Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates . Recombinant protein expressed in E. coli thus retains all known biological activities.The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host- virus interaction.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
IL-10 in disease
IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni, Leishmania, Toxoplasma gondii and Trypanosoma infection. Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae, Mycobacterium tuberculosis and Mycobacterium avium infections. High expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency.
Version 9 - 09.22
For research use only
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