Human Granzyme B ELISA kit

Product Specifications

Catalogue N°

850.790.048 - 1 x 48 Discovery (pre-coated)
850.790.096 - 1 x 96 (pre-coated)
850.790.192 - 2 x 96 (pre-coated)

Assay Range

31.25 pg/ml - 1000 pg/ml

Sensitivity

20 pg/ml

Target species

Human

Specificity

Recognizes both natural and recombinant human Granzyme B

Incubation

3h45

Sample Type

Serum, Plasma, Cell culture supernatant

Sample Size

100 µl

Cross Reaction

No cross reactivity with Trail, TNFα, IFNγ, Fas, IL-1α, IL-4, IL-6, IL-8, IL-13

Kit Content

Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.

• References
• Related products

BACKGROUND

Granzyme B belongs to the granzymes family, which are exogenous serine proteinases. Granzymes are produced by cytotoxic lymphocytes (CTLs) and NK cells.

Activated cytotoxic T lymphocytes and NK cells are important effectors in immunologic defenses against tumor cell proliferation, viral infection and transplant tissue. They induce apoptosis via multiple mechanism thanks to granules exocytosis. Following adhesion of the CTL on the target cell (via CTL-receptor and antigen-presenting MHC molecules), CTLs secrete cytoplasmic granules containing granules and enzymes including granzymes and perforine (pore-forming protein). Granules are then released in the intercellular space, perforine forms a transmembrane pore. Through these pores, granzymes enter the cytosol of the target cell.

Granzyme B is the most powerful pro-apoptotic member of granzyme family. Granzyme B activates the intracellular caspases induising finally the death of the cell.

Granzymes are diagnostics markers for many disorders (viral infection, lymphoma’s and carcinoma’s, auto-immune disease and rejection of transplant).

Version 14 - 02.22

For research use only

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