Search for products

Human CD62E ELISA kit

Product Specifications

Catalogue N°
850.530.048 - 1 x 48 Discovery (pre-coated)
850.530.096 - 1 x 96 (pre-coated)
850.530.192 - 2 x 96 (pre-coated)
Assay Range
1 ng/ml - 32 ng/ml
Sensitivity
0.5 ng/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human soluble ELAM-1
Incubation
2h15
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human adhesion molecules
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
ELAM-1
E-Selectin
CD62E ELISA Kit
Example of Human CD62E Standard Curve

BACKGROUND

E-selectin (CD62E) is a 115 kDa glycoprotein, expressed only on endothelial cells after activation with interleukin-1 (IL-1), tumor necrosis factor-α (TNFα), or bacterial lipopolysaccharides. After stimulation of the endothelial cells, the newly synthesized E-selectin is rapidly detected, with maximum surface expression after 3-6 h and return to basal levels within 24 h. This rapid down-regulation, although poorly understood, has been explained by the release of a soluble form of E-selectin and the internalization of the molecule. This regulation of E-selectin expression could be crucial for controlling leukocyte accumulation in inflammatory responses. Several E-selectin ligands have been identified on leukocytes, but have not yet been cloned. Monoclonal antibodies (mAbs) specific for E selectin have been shown to inhibit leukocyte transmigration. It has been suggested that leukocyte binding to E-selectin on activated endothelium positively regulates CD11b (Mac-1) on leukocytes and induces increased adhesion by the ICAM-1 / Mac-1 interaction.

Version 21 - 09.22

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

850.530.048 1 x 48 Discovery (pre-coated)
850.530.096 1 x 96 (pre-coated)
850.530.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human CD62E ELISA kit 850.530.048 - 1 x 48 Discovery (pre-coated) 850.530.096 - 1 x 96 (pre-coated) 850.530.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders