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Human IL-4 ELISpot Set

Product Specifications

Catalogue N°
856.011.001 - 1 x 96 Discovery (plate not included)
856.011.001P - 1 x 96 Discovery (non-sterile plate)
856.011.001S - 1 x 96 Discovery (sterile plate)
856.011.005 - 5 x 96 (plates not included)
856.011.005P - 5 x 96 (non-sterile plates)
856.011.005S - 5 x 96 (sterile plates)
856.011.010 - 10 x 96 (plates not included)
856.011.010P - 10 x 96 (non-sterile plates)
856.011.010S - 10 x 96 (sterile plates)
856.011.015 - 15 x 96 (plates not included)
856.011.015P - 15 x 96 (non-sterile plates)
856.011.015S - 15 x 96 (sterile plates)
856.011.020 - 20 x 96 (plates not included)
856.011.020P - 20 x 96 (non-sterile plates)
856.011.020S - 20 x 96 (sterile plates)
Target species
Human
Specificity
Recognizes natural human IL-4
Incubation
3h after cell stimulation
Cross Reaction
No cross reactivity with other human cytokines
Kit Content
Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB and blocking reagent.
Additional Information
Spots read visually or with reader

BACKGROUND

IL-4 is a lymphokine that co-stimulates the proliferation of activated B- and T-cells, augments the cytotoxic activity of lymphocytes and monocytes and enhances the functional activity of myeloid cells. Produced by mast cells, T-cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ cells into helper Th2 cells characterized by their cytokine secretion-profile that includes secretion of IL-4, IL-5, IL-6, IL-10 and IL-13 which favour a humoral immune response. In addition, IL-4 can inhibit the proliferation of TNF, IL-1 and IL-6 by macrophages.

IL-4 induces B-cell class switching to IgE and IgG1 isotypes, and up regulates MHC Class II production and CD23 expression.

IL-4 is a 15kDa globular glycoprotein containing 129 amino acid residues. The non-glycosylated form of the protein is fully biologically active.

Version 9 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

856.011.001 1 x 96 Discovery (plate not included)
856.011.001P 1 x 96 Discovery (non-sterile plate)
856.011.001S 1 x 96 Discovery (sterile plate)
856.011.005 5 x 96 (plates not included)
856.011.005P 5 x 96 (non-sterile plates)
856.011.005S 5 x 96 (sterile plates)
856.011.010 10 x 96 (plates not included)
856.011.010P 10 x 96 (non-sterile plates)
856.011.010S 10 x 96 (sterile plates)
856.011.015 15 x 96 (plates not included)
856.011.015P 15 x 96 (non-sterile plates)
856.011.015S 15 x 96 (sterile plates)
856.011.020 20 x 96 (plates not included)
856.011.020P 20 x 96 (non-sterile plates)
856.011.020S 20 x 96 (sterile plates)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human IL-4 ELISpot Set 856.011.001 - 1 x 96 Discovery (plate not included) 856.011.001P - 1 x 96 Discovery (non-sterile plate) 856.011.001S - 1 x 96 Discovery (sterile plate) 856.011.005 - 5 x 96 (plates not included) 856.011.005P - 5 x 96 (non-sterile plates) 856.011.005S - 5 x 96 (sterile plates) 856.011.010 - 10 x 96 (plates not included) 856.011.010P - 10 x 96 (non-sterile plates) 856.011.010S - 10 x 96 (sterile plates) 856.011.015 - 15 x 96 (plates not included) 856.011.015P - 15 x 96 (non-sterile plates) 856.011.015S - 15 x 96 (sterile plates) 856.011.020 - 20 x 96 (plates not included) 856.011.020P - 20 x 96 (non-sterile plates) 856.011.020S - 20 x 96 (sterile plates) )

Please contact us to discuss bulk orders